The Journal of Urology
Volume 184, Issue 2 , Pages 757-761 , August 2010

Minced Urothelium to Create Epithelialized Subcutaneous Conduits

  • Magdalena Fossum

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • Department of Women's and Children's Health, Astrid Lindgren Children's Hospital, Karolinska Institutet, Stockholm, Sweden
    • Corresponding Author InformationCorrespondence: Division of Pediatric Urology, Astrid Lindgren Children's Hospital, Q3:03, Karolinska Institutet, SE 171 76 Stockholm, Sweden (telephone: +46 8 517 783 10; FAX: +46 8 517 777 12)
    • ,
  • Baraa Zuhaili

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • ,
  • Juri Bergmann

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • Current address: Steinwies Str. 75, 8032 Zürich, Switzerland.
    • ,
  • Malte Spielmann

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • ,
  • Tobias Hirsch

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • Current address: Klinik für Plastische Chirurgie und Schwerbrandverletzte, BG Universitätskliniken Bergmannsheil, Ruhr Universität Bochum, Buerkle-de-la Camp Platz 1, D-44789 Bochum, Germany.
    • ,
  • Elof Eriksson

      Affiliations

    • Laboratory of Wound Healing and Gene Transfer, Division of Plastic Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts
    • Current address: Klinik für Plastische Chirurgie und Schwerbrandverletzte, BG Universitätskliniken Bergmannsheil, Ruhr Universität Bochum, Buerkle-de-la Camp Platz 1, D-44789 Bochum, Germany.

Received 13 October 2009

References 

  1. Atala A, Bauer SB, Soker S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet. 2006;367:1241
  2. Fossum M, Svensson J, Kratz G, et al. Autologous in vitro cultures urothelium in hypospadias repair. J Pediatr Urol. 2006;3:10
  3. Fossum M, Zuhaili B, Hirsch T, et al. Minced skin for tissue engineering of epithelialized subcutaneous tunnels. Tissue Eng Part A. 2009;15:2085
  4. Fossum M and Nordenskjöld A: A long-term follow-up after hypospadias repair with cultured autologous urothelial cells. Unpublished data.
  5. Wood D, Southgate J. Current status of tissue engineering in urology. Curr Opin Urol. 2008;18:564
  6. Fossum M, Gustafson CJ, Nordenskjöld A, et al. Isolation and in vitro cultivation of human urothelial cells from bladder washings of adult patients and children. Scand J Plast Reconstr Surg Hand Surg. 2003;37:41
  7. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975;6:331
  8. Green H, Rheinwald JG, Sun TT. Properties of an epithelial cell type in culture: the epidermal keratinocyte and its dependence on products of the fibroblast. Prog Clin Biol Res. 1977;17:493
  9. Lysaght MJ, Hazlehurst AL. Tissue engineering: the end of the beginning. Tissue Eng. 2004;10:309
  10. Meek CP. Successful microdermagrafting using the Meek-Wall microdermatome. Am J Surg. 1958;96:557
  11. Vranckx JJ, Slama J, Preuss S, et al. Wet wound healing. Plast Reconstr Surg. 2002;110:1680
  12. Vogt PM, Andree C, Breuing K, et al. Dry, moist, and wet skin wound repair. Ann Plast Surg. 1995;34:493
  13. De Queiroz M, Combet S, Bérard J, et al. Latex allergy in children: modalities and prevention. Paediatr Anaesth. 2009;19:313

 Study received approval from the Harvard Medical Area Standing Committee on Animals.

 Supported by the Sweden-America Foundation, Karolinska Institutet, Stockholm County Council, Swedish Society for Medical Research and National Institutes of Health Grant 5ROIGM51449.

PII: S0022-5347(10)03390-2

doi: 10.1016/j.juro.2010.04.031

The Journal of Urology
Volume 184, Issue 2 , Pages 757-761 , August 2010

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