The Journal of Urology
Volume 183, Issue 1 , Pages 351-356 , January 2010

O-GlcNAc Mediated Glycosylation Down-Regulation in Mice With Cyclophosphamide Induced Cystitis

  • Seyung Chung

      Affiliations

    • Developmental Biology, Regenerative Medicine and Surgery Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California
    • Equal study contribution.
  • ,
  • Dae-Ook Kang

      Affiliations

    • Department of Biochemistry and Health Science, Changwon National University, Changwon, South Korea
    • Equal study contribution.
  • ,
  • Jonathan Yamzon

      Affiliations

    • Department of Urology, University of Southern California Keck School of Medicine, Los Angeles, California
  • ,
  • David Warburton

      Affiliations

    • Developmental Biology, Regenerative Medicine and Surgery Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California
  • ,
  • Chester J. Koh

      Affiliations

    • Los Angeles, California
    • Corresponding Author InformationCorrespondence: Children's Hospital Los Angeles, 4650 Sunset Blvd., Mailstop 114, Los Angeles, California 90027 (telephone: 323-361-2247; FAX: 323-361-8034)

Received 30 March 2009

References 

  1. Ferrero-Miliani L, Nielsen OH, Andersen PS, et al. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007;147:227
  2. Gornik O, Lauc G. Glycosylation of serum proteins in inflammatory diseases. Dis Markers. 2008;25:267
  3. Ohtsubo K, Marth JD. Glycosylation in cellular mechanisms of health and diseases. Cell. 2006;126:855
  4. Halbleib JM, Nelson WJ. Cadherins in development. Genes and Dev. 2006;20:3199
  5. Campbell BJ, Yu LG, Rhodes JM. Altered glycosylation in inflammatory bowel disease: a possible role in cancer development. Glycoconj J. 2001;18:851
  6. Slawson C, Pidala J, Potter R. Increased N-acetyl-beta-glucosaminidase activity in primary breast carcinomas corresponds to a decrease in N-acetylglucosamine containing proteins. Biochim Biophys Acta. 2001;1537:147
  7. Hart GW, Housley MP, Slawson C. Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins. Nature. 2007;446:1017
  8. Lubas WA, Hanover JA. Functional expression of O-linked GlcNAc transferase (Domain structure and substrate specificity). J Biol Chem. 2000;275:10983
  9. Shanafelt TD, Lin T, Geyer SM. Pentostatin, cyclophosphamide, and rituximab regimen in older patients with chronic lymphocytic leukemia. Cancer. 2007;109:2291
  10. Ahluwalia A, Maggi CA, Santicioli P, et al. Characterisation of the capsaicin-sensitive component of cyclophosphamide-induced inflammation in the rat urinary bladder. Br J Pharmacol. 1994;111:1017
  11. Vizzard MA. Up-regulation of pituitary adenylate cyclase-activating polypeptide in urinary bladder pathways after chronic cystitis. J Comp Neurol. 2000;420:335
  12. Yoshimura N, de Groat WC. Increased excitablity of afferent neurons innervating rat urinary bladder following chronic bladder inflammation. J Neurosci. 1999;19:4644
  13. Vizzard MA. Changes in urinary bladder neurotrophic factor mRNA and NGF protein following urinary bladder dysfunction. Exp Neurol. 2000;161:273
  14. Murray E, Malley SE, Li-Ya Q, et al. Cyclophosphamide induced cystitis alters neurotrophin and receptor tyrosine kinase expression in pelvic ganglia and bladder. J Urol. 2004;172:2434
  15. Jezernik K, Romih R, Mannherz HG. Immunohistochemical detection of apoptosis, proliferation and inducible nitric oxide synthase in rat urothelium damaged by cyclophosphamide treatment. Cell Biol Int. 2003;27:863
  16. Farsund T. Cell kinetics of mouse urinary bladder epithelium. II. Changes in proliferation and nuclear DNA content during necrosis regeneration, and hyperplasia caused by a single dose of cyclophosphamide. Virchows Arch. 1976;21:279
  17. Kunze E, Köhnecke B, Engelhardt W. Effect of the uroprotector sodium 2-mercaptoethane sulfonate (Mesna) on the proliferation of the bladder urothelium in the rat after administration of cyclophosphamide. Urol Int. 1984;39:61
  18. Zupanĉiĉ D, Jezernik K, Vidmar G. Effect of melatonin on apoptosis, proliferation and differentiation of urothelial cells after cyclophosphamide treatment. J Pineal Res. 2008;44:299
  19. Golks A, Guerini D. The O-linked N-acetylglucosamine modification in cellular signalling and the immune system (Protein modifications: beyond the usual suspects' review series). EMBO Rep. 2008;9:748
  20. Yang X, Zhang F, Kudlow JE. Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A; coupling protein O-GlcNAc transferase to transcriptional repression. Cell. 2002;110:69
  21. O'Donnell N, Zachara NE, Hart GW, et al. Ogt-dependent X-Chromosome linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol Cell Biol. 2004;24:1680
  22. Slawson C, Pidala J, Potter R. Increased N-acetyl-b-glucosaminidase activity in primary breast carcinoma corresponds to a decrease in N-acetylglucosamine containing protein. Biochim Biophys Acta. 2001;1537:147
  23. Xing D, Feng W, Not LG, et al. Increased protein O-GlcNAc modification inhibits inflammatory and neointimal responses to acute endoluminal arterial injury. Am J Physiol Heart Circ Physiol. 2008;295:335
  24. Dube DH, Bertozzi CR. Glycans in cancer and inflammation—potential for therapeutics and diagnostics. Nat Rev Drug Discov. 2005;4:477

 Study received approval from the Children's Hospital Los Angeles institutional animal care and use committee.

 Supported by National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases 5K08DK078589-2, an American College of Surgeons Faculty Research Fellowship and a Spina Bifida Association research grant.

PII: S0022-5347(09)02315-5

doi: 10.1016/j.juro.2009.08.102

The Journal of Urology
Volume 183, Issue 1 , Pages 351-356 , January 2010