Proteomics and Opportunities for Clinical Translation in Urological Disease
Purpose
Proteomics is a rapidly growing new discipline that has the potential to increase and improve the understanding of protein function and interaction in the context of systems biology. As a translational science it has the potential to identify many new therapeutic targets as well as diagnostic and prognostic biomarkers of disease. Proteomics approaches consist of a combination of powerful technologies such as protein/peptide separation, identification and bioinformatic detection, and quantitation based on powerful computational data processing tools. We present an overview of current proteomics technologies, a review of proteomics applications in urology and a perspective on the future of proteomics in clinical medicine.
Materials and Methods
A literature search was performed on the basic concepts of proteomics and technologies commonly used in this field. Advantages, challenges and limitations of current proteomics approaches are discussed, and proteomics applications in the field of urology are presented.
Results
The proteomics approaches to answer clinical questions have only recently been introduced. Many different technologies have been used in this field, which is moving from simple description to quantitative clinical applications.
Conclusions
Proteomics offers new approaches to the study of genitourinary tract diseases, and the potential to identify clinically relevant biomarkers and new therapeutic targets.
Key Words: mass spectrometry, chromatography, liquid, biological markers, proteomics
Abbreviations and Acronyms: 2-DE, 2-dimensional gel electrophoresis, CE, capillary electrophoresis, IEF, isoelectric focusing, LC, liquid chromatography, LCM, laser capture microdissection, m/z, mass-to-charge ratio, MALDI, matrix-assisted laser desorption ionization, MRM, multiple reaction monitoring, MS, mass spectrometry, MS/MS, tandem mass spectra, PTM, post-translational modification, RP, reverse phase, SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SELDI, surface enhanced laser desorption ionization, SILAC, stable isotope labeling with amino acids in cell culture, TOF, time of flight
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Supported by NIH P50DK65298 (MRF), NIH K08DK077836 (RSL) and a Children's Hospital Boston Career Development Fellowship (RSL).
The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
PII: S0022-5347(09)01138-0
doi:10.1016/j.juro.2009.05.001
© 2009 American Urological Association. Published by Elsevier Inc. All rights reserved.
