The Journal of Urology
Volume 182, Issue 2 , Pages 741-748 , August 2009

DNA Microarray Expression Profiling of Bladder Cancer Allows Identification of Noninvasive Diagnostic Markers

  • Lourdes Mengual

      Affiliations

    • Laboratory of Urology, Urology Department, Hospital Clínic, Barcelona, Spain
    • Corresponding Author InformationCorrespondence: Laboratory of Urology, Urology Department, Hospital Clínic, Villarroel, 170, 08036 Barcelona, Spain (telephone: 00 34 93 2275545; FAX: 00 34 93 2275545)
    • Equal study contribution.
  • ,
  • Moisès Burset

      Affiliations

    • Laboratory of Urology, Urology Department, Hospital Clínic, Barcelona, Spain
    • Equal study contribution.
  • ,
  • Elisabet Ars

      Affiliations

    • Laboratory of Molecular Biology and Service of Urology, Fundació Puigvert, Barcelona, Spain
  • ,
  • Juan José Lozano

      Affiliations

    • Ciber de Enfermedades Hepáticas y Digestivas, Hospital Clínic, Barcelona, Spain
    • Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
  • ,
  • Humberto Villavicencio

      Affiliations

    • Laboratory of Molecular Biology and Service of Urology, Fundació Puigvert, Barcelona, Spain
  • ,
  • María José Ribal

      Affiliations

    • Laboratory of Urology, Urology Department, Hospital Clínic, Barcelona, Spain
    • Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
  • ,
  • Antonio Alcaraz

      Affiliations

    • Laboratory of Urology, Urology Department, Hospital Clínic, Barcelona, Spain
    • Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain

Received 14 November 2008

References 

  1. Landis SH, Murray T, Bolden S, et al. Cancer statistics, 1999. CA Cancer J Clin. 1999;49:8
  2. Amira N, Mourah S, Rozet F, et al. Non-invasive molecular detection of bladder cancer recurrence. Int J Cancer. 2002;101:293
  3. van Rhijn BW, van der Poel HG, van der Kwast TH. Urine markers for bladder cancer surveillance: a systematic review. Eur Urol. 2005;47:736
  4. Sanchez-Carbayo M, Socci ND, Charytonowicz E, et al. Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes. Cancer Res. 2002;62:6973
  5. Dyrskjot L, Thykjaer T, Kruhoffer M, et al. Identifying distinct classes of bladder carcinoma using microarrays. Nat Genet. 2003;33:90
  6. Thykjaer T, Workman C, Kruhoffer M, et al. Identification of gene expression patterns in superficial and invasive human bladder cancer. Cancer Res. 2001;61:2492
  7. Dyrskjot L, Zieger K, Real FX, et al. Gene expression signatures predict outcome in non-muscle-invasive bladder carcinoma: a multicenter validation study. Clin Cancer Res. 2007;13:3545
  8. Mengual L, Burset M, Ars E, et al. Partially degraded RNA from bladder washing is a suitable sample for studying gene expression profiles in bladder cancer. Eur Urol. 2006;50:1347
  9. Lopez-Beltran A, Sauter G, Gasser T, et al. Tumours of the urinary system. In:  Eble JN,  Sauter G,  Epstein JI,  Sesterhenn IA editor. Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs (World Health Organization Classification of Tumours). Lyon: IARC Press; 2004;p. 89–157chapt 2
  10. Sobin LH, Wittekind CH. TNM Classification of Malignant Tumours. In: International Union Against Cancer. 6th ed.. New York: Wiley-Liss Publications; 2002;
  11. Peng X, Wood CL, Blalock EM, et al. Statistical implications of pooling RNA samples for microarray experiments. BMC Bioinform. 2003;4:26
  12. Irizarry RA, Bolstad BM, Collin F, et al. Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Res. 2003;31:e15
  13. Herrero J, Al Shahrour F, Diaz-Uriarte R, et al. GEPAS: a web-based resource for microarray gene expression data analysis. Nucleic Acids Res. 2003;31:3461
  14. Henderson AR. The bootstrap: a technique for data-driven statistics (Using computer-intensive analyses to explore experimental data). Clin Chim Acta. 2005;359:1
  15. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods. 2001;25:402
  16. Sanchez-Carbayo M, Socci ND, Lozano JJ, et al. Gene discovery in bladder cancer progression using cDNA microarrays. Am J Pathol. 2003;163:505
  17. Gunlusoy B, Degirmenci T, Arslan M, et al. Recurrence and progression of T1G3 transitional cell carcinoma of the bladder treated with intravesical bacillus Calmette-Guerin. Urol Int. 2005;75:107
  18. Blaveri E, Simko JP, Korkola JE, et al. Bladder cancer outcome and subtype classification by gene expression. Clin Cancer Res. 2005;11:4044
  19. Sotiriou C, Neo SY, McShane LM, et al. Breast cancer classification and prognosis based on gene expression profiles from a population-based study. Proc Natl Acad Sci U S A. 2003;100:10393
  20. Holyoake A, O'Sullivan P, Pollock R, et al. Development of a multiplex RNA urine test for the detection and stratification of transitional cell carcinoma of the bladder. Clin Cancer Res. 2008;14:742

 Study received hospital ethics committee approval.

 Supported by Laboratorios Fina Biotech, Spanish Urological Association Grant FIU 2007 and Fondo de Investigaciones Sanitarias Grant PI070040.

 Supplementary material for this article can be obtained at http://bioinfo.ciberehd.org/mengual_et_al_2009/.

PII: S0022-5347(09)00884-2

doi: 10.1016/j.juro.2009.03.084

The Journal of Urology
Volume 182, Issue 2 , Pages 741-748 , August 2009