The Journal of Urology
Volume 175, Issue 2 , Pages 495-499 , February 2006

Virtual Microscopy in Prostate Histopathology: Simultaneous Viewing of Biopsies Stained Sequentially With Hematoxylin and Eosin, and α-Methylacyl-Coenzyme A Racemase/p63 Immunohistochemistry

  • Henrik O. Helin

      Affiliations

    • Institute of Medical Technology, University of Tampere, Tampere
    • ,
  • Mikael E. Lundin

      Affiliations

    • Biomedical Informatics Group, HUSLAB, Helsinki University Central Hospital, Helsinki
    • ,
  • Mervi Laakso

      Affiliations

    • Department of Pathology, Seinäjoki Central Hospital, Seinäjoki, Finland
    • ,
  • Johan Lundin

      Affiliations

    • Biomedical Informatics Group, HUSLAB, Helsinki University Central Hospital, Helsinki
    • ,
  • Heikki J. Helin

      Affiliations

    • Department of Oncology, University of Helsinki and Division of Pathology, HUSLAB, Helsinki University Central Hospital, Helsinki
    • ,
  • Jorma Isola

      Affiliations

    • Institute of Medical Technology, University of Tampere, Tampere
    • Corresponding Author InformationCorrespondence: Institute of Medical Technology, 33014 University of Tampere, Tampere, Finland (telephone: +358-3-2156729; FAX: +358-3-2158923)

Received 17 February 2005

References 

  1. Epstein JI , Algaba F , Allsbrook WC , Bastacky S , Boccon-Gibond L , De Marzo AM , et al.   Acinar adenocarcinoma . In:  Eble JN ,  Sauter G ,  Epstein JI ,  Sesterhenn IA editor. WHO Classification of Tumours (Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs) . Lyon: IARC Press; 2004;
  2. Epstein JI . Diagnosis and reporting of limited adenocarcinoma of the prostate on needle biopsy . Mod Pathol . 2004;17:307
  3. Van der Kwast TH , Lopes C , Martikainen PM , Pihl CG , Santonja C , Neetens I , et al.   Report of the Pathology Committee (false-positive and false-negative diagnoses of prostate cancer) . BJU Int . 2003;92(suppl):62
  4. Hedrick L , Epstein JI . Use of keratin 903 as an adjunct in the diagnosis of prostate carcinoma . Am J Surg Pathol . 1989;13:389
  5. O’Malley FP , Grignon DJ , Shum DT . Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland . Virchows Arch A Pathol Anat Histopathol . 1990;417:191
  6. Shin M , Fujita MQ , Yasunaga Y , Miki T , Okuyama A , Aozasa K . Utility of immunohistochemical detection of high molecular weight cytokeratin for differential diagnosis of proliferative conditions of the prostate . Int J Urol . 1998;5:237
  7. Signoretti S , Waltregny D , Dilks J , Isaac B , Lin D , Garraway L , et al.   p63 is a prostate basal cell marker and is required for prostate development . Am J Pathol . 2000;157:1769
  8. Parsons JK , Gage WR , Nelson WG , De Marzo AM . p63 protein expression is rare in prostate adenocarcinoma (implications for cancer diagnosis and carcinogenesis) . Urology . 2001;58:619
  9. Shah RB , Zhou M , LeBlanc M , Snyder M , Rubin MA . Comparison of the basal cell-specific markers, 34betaE12 and p63, in the diagnosis of prostate cancer . Am J Surg Pathol . 2002;26:1161
  10. Weinstein MH , Signoretti S , Loda M . Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells . Mod Pathol . 2002;15:1302
  11. Xu J , Stolk JA , Zhang X , Silva SJ , Houghton RL , Matsumura M , et al.   Identification of differentially expressed genes in human prostate cancer using subtraction and microarray . Cancer Res . 2000;60:1677
  12. Evans AJ . Alpha-methylacyl CoA racemase (P504S) (overview and potential uses in diagnostic pathology as applied to prostate needle biopsies) . J Clin Pathol . 2003;56:892
  13. Tacha DE , Miller RT . Use of p63/P504S monoclonal antibody cocktail in immunohistochemical staining of prostate tissue . Appl Immunohistochem Mol Morphol . 2004;12:75
  14. Molinie V , Fromont G , Sibony M , Vieillefond A , Vassiliu V , Cochand-Priollet B , et al.   Diagnostic utility of a p63/alpha-methyl-CoA-racemase (p504s) cocktail in atypical foci in the prostate . Mod Pathol . 2004;17:1180
  15. Dardik M , Epstein JI . Efficacy of restaining prostate needle biopsies with high-molecular weight cytokeratin . Hum Pathol . 2000;31:1155
  16. Green R , Epstein JI . Use of intervening unstained slides for immunohistochemical stains for high molecular weight cytokeratin on prostate needle biopsies . Am J Surg Pathol . 1999;23:567
  17. Kubier P , Miller RT . Tissue protection immunohistochemistry (a useful adjunct in the interpretation of prostate biopsy specimens and other selected cases in which immunostains are needed on minute lesions) . Am J Clin Pathol . 2002;117:194
  18. Lundin M , Lundin J , Isola J . Virtual microscopy . J Clin Pathol . 2004;57:1250
  19. Helin H , Lundin M , Lundin J , Martikainen P , Tammela T , Helin H , et al.   Web-based virtual microscopy in teaching and standardizing Gleason grading . Hum Pathol . 2005;36:381
  20. Lundin M , Lundin J , Helin H , Isola JA . digital atlas of breast histopathology (an application of web-based virtual microscopy) . J Clin Pathol . 2004;57:1288

 Supported by grants from the Scientific Foundation of Instrumentarium, Inc., Finska Läkaresällskapet, Biomedicum Foundation, Medicinska Understödsföreningen Liv och Hälsa, Svenska Kulturfonden, Finnish Cancer Foundation and Sigfrid Juselius Foundation.

PII: S0022-5347(05)00164-3

doi: 10.1016/S0022-5347(05)00164-3

The Journal of Urology
Volume 175, Issue 2 , Pages 495-499 , February 2006

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